Table 9.

Immune parameters, oxidative stress, morphology, crypt cell proliferation, and expression of tight junction protein genes in the jejunum of nursery pigs fed control or LCF diets¹ with or without β-mannanase

Diet1	Control	LCF			P-value
M2	−	−	+	SEM	Diet	M
Inflammation and oxidative stress3
IL-6, pg/mg	10.46	12.66	10.58	1.98	0.239	0.324
IL-8, ng/mg	0.45	0.43	0.45	0.02	0.504	0.502
TNF-α, pg/mg	2.02	2.20	2.19	0.32	0.543	0.915
MDA, nmol/mg	0.30a	0.20b	0.27ab	0.04	0.097	0.232
PC, nmol/mg	1.18	1.02	1.07	0.14	0.380	0.766
Morphology and proliferation3
VH, µm	457.3	447.4	474.1	26.4	0.794	0.486
CD, µm	199.1	210.2	213.9	11.2	0.497	0.818
VH:CD	2.30	2.13	2.22	0.11	0.134	0.379
Ki-67+, %	18.50	21.63	20.82	2.02	0.220	0.742
Genes4
ZO-1	1.09a	0.76b	0.84ab	0.13	0.098	0.721
OC	0.91	0.75	0.91	0.09	0.249	0.223
CL-1	1.45	0.98	1.33	0.65	0.309	0.236
MUC-1	1.03	1.08	1.14	0.27	0.921	0.873
MUC-2	1.06	1.41	1.29	0.20	0.233	0.692

¹Control and low-cost formulated feed (LCF).

²Mannanase supplemented at 100 g/t, supplying 800 TMU/kg.

³Ki-67⁺, ratio of Ki-67-positive cells to total cells in the crypt.

⁴ZO-1, zona occludens; OC, occuldin; CL-1, claudin-1; MUC-1, mucin-1; MUC-2, mucin-2.

^abWithin a row, values with different superscripts are significantly different at P < 0.05.