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. 2024 Jun 20;6(1):vdae104. doi: 10.1093/noajnl/vdae104

Figure 4.

Figure 4.

Response to MLN4924 is independent of PTEN lipid phosphatase activity but dependent on phosphorylation of PTEN Tyrosine 240. (A) Protein lysates from LN18, GB1, M059K, and SNU1105 were probed for phosphorylated- and total-AKT (pAKT and AKT) by IB analysis, and GAPDH loading control. (B) Viability of Transgene PTEN WT or PTEN G129A (phosphatase-dead) in PTEN-null GSC23 cells after treatment with MLN4924. (C) Viability of PTEN WT or PTEN Y240F mouse embryonic fibroblasts (MEFs) after treatment with MLN4924. B and C are in 5 replicates. (D) Volcano plot of differentially expressed transcripts between isogenic MEFs from Figure 4C (listed in Supplementary Table 3). (E) Waterfall plot (ranked by fold enrichment) of top 100 Reactome pathways from data in Figure 4D. Cell cycle and DNA replication pathways (n = 23) are annotated as "TRUE"; 10 characteristic pathways are labeled for clarity.