FIG. 2.

Northern analysis of kinetics of expression of endogenous genes of human endothelial cells following infection by an E1⁻ E4⁺ Ad vector. (A) Control, uninfected cells. Total RNA (10 μg/lane) was isolated from uninfected control cells over time (6 to 72 h). Each lane was then hybridized to a specific probe as indicated. Note that, other than the 4.0-kb transcript of TGF-β2, there is little change in the transcripts. (B) Cells infected with an E1⁻ E4⁺ Ad vector. Total RNA was assessed as in panel A. In contrast to panel A, there are marked changes in all transcripts other than GAPDH. The GAPDH probe was used to confirm the integrity and equal loading of RNA.