Fig. 2.

High glucose (HG) promoted the transformation of cardiac fibroblasts (CFs) into myofibroblasts and reduced the phosphorylation of enhancer of zeste homolog 2 (EZH2) at T311. (A) Protein levels of extracellular matrix-related genes and α-smooth muscle actin (α-SMA) were analyzed in primary CFs after HG treatment (30 mM) for 72 hours (n=3 in each group). (B) Representative pictures of immunofluorescence of α-SMA expression in CFs (red, α-SMA; blue, 4´,6-diamidino-2-phenylindole [DAPI]; scale bar 50 μm). (C) Transwell assays were performed on CFs under HG treatment for 72 hours (scale bar 200 μm, n=3 in each group). (D) Scratching tests were performed on CFs under HG treatment for 72 hours (scale bar 400 μm, n=4 in each group). (E) Western blot analysis of the expression of phospho-EZH2 (p-EZH2), EZH2, and histone H3 lysine 27 trimethylation (H3K27me3) in CFs under HG treatment for 72 hours (n=3 in each group). Data are presented as mean±standard error of the mean. TGF-β, transforming growth factor-β; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NG, normal glucose. ^aP<0.05, ^bP<0.01.