Table 1.
Characterization and validation.
| Classification | Test | Result | Data |
|---|---|---|---|
| Morphology | Photography Bright field | Normal morphology of human iPS cells | Fig. 1 A |
| Phenotype | Qualitative analysis by Immunocytochemistry |
hiPS cells express stemness markers: Oct4, Nanog, Tra 1–60, SSEA-4. | Fig. 1 B |
| Qualitative analysis by RT-qPCR |
Expression of stemness markers compared to ACS-1019 (CTRL) reference cell line/parental fibroblasts | Fig. 1 C | |
| Genotype | hPSC Genetic Analysis Kit; CGH array (60 kb) | No abnormalities detected. 46, XX. No abnormalities detected |
Fig. 1 D; Suppl. Fig. 1 B |
|
Identity |
Microsatellite PCR (mPCR) OR | Not performed | N/A |
| STR analysis | 16 markers tested- matched | submitted in archive with journal | |
|
Mutation analysis |
Sequencing | heterozygous, type of mutation. REEP1 (c.337C > T; p.Arg113Ter) | Fig. 1 E |
| Southern Blot OR WGS | N/A | N/A | |
| Microbiology and virology | Mycoplasma detection by PCR | Negative | Fig. 1 F |
| Differentiation potential | Directed differentiation | Expression of layer-specific markers: Ectoderm (Nestin, β3- tubulin), Mesoderm (BRACHYURY) and Endoderm (SOX17). | Fig. 1 G |
| Donor screening (OPTIONAL) | HIV 1 + 2 Hepatitis B, Hepatitis C | N/A | N/A |
| Genotype additional info (OPTIONAL) | Blood group genotyping | N/A | N/A |
| HLA tissue typing | N/A | N/A |