Table 2.

Reagents details.

	Antibodies used for immunocytochemistry
	Antibody	Dilution	Company Cat #
Stemness Markers	Rabbit anti-OCT4 Rabbit anti-Nanog (D73G4) Mouse anti-TRA-1–60 Mouse anti-SSEA4 (clone MC813)	1:500 1:200 1:500 1:500	Abcam Cat# ab19857 Cell Signaling Technology Cat# 4903 Cell Signaling Technology Cat # 4746 Cell Signaling Technology Cat #4755
Differentiation Markers	Mouse anti-Nestin Mouse anti-Beta-3 Tubulin Rabbit anti-Brachyury (D2Z3J) Rabbit anti-SOX17 (D1T8M)	1:500 1:1000 1:500 1:500	Abcam Cat# ab18102 Abcam Cat# ab7751 Cell Signaling Cat# 81694 Cell Signaling Cat# 81778
Secondary antibodies	Goat Anti-Mouse Alexa Fluor 488 Goat Anti-Rabbit Alexa Fluor 555	1:500 1:500	Thermo Fisher Scientific Cat# a11029 Thermo Fisher Scientific Cat# a21429
	Primers
	Target	Size of band	Forward/Reverse primer (5′-3′)
Episomal Plasmids (qPCR)	eOCT4 eLIN28 eSOX2 eKLF4	70–150 bp 70–150 bp 70–150 bp 70– 150 bp	Fwd: CAT TCA AAC TGA GGT AAG GG Rev: TAG CGT AAA AGG AGC AAC ATA G Fwd: AGC CAT ATG GTA GCC TCA TGT CCG C Rev: TAG CGT AAA AGG AGC AAC ATA G Fwd: TTC ACA TGT CCC AGC ACT ACC AGA Rev: TTT GTT TGA CAG GAG CGA CAA T Fwd: CCA CCT CGC CTT ACA CAT GAA GA Rev: TAG CGT AAA AGG AGC AAC ATA G
Stemness Markers (qPCR)	OCT4 L-MYC SOX2	70–150 bp 70–150 bp 70–150 bp	Fwd: CCC CAG GGC CCC ATT TTG GTA CC Rev: ACC TCA GTT TGA ATG CAT GGG AGA GC Fwd: GCG AAC CCA AGA CCC AGG CCT GCT CC Rev: CAG GGG GTC TGC TCG CAC CGT GAT G Fwd: TTC ACA TGT CCC AGC ACT ACC AGA Rev: TCA CAT GTG TGA GAG GGG CAG TGT GC
House-Keeping Genes (qPCR)	GAPDH	70–150 bp	Fwd: GGA AGG ACT CAT GAC CAC AGT Rev: GGA TGA TGT TCT GGA GAG CCC
Genotyping	chr2:86,251,853–86,252,186 (spanning REEP1 Exon 5)	334 bp	Fwd: CCC AGC AAG AAC AAG GAT TG Rev: CCA CTG ATT GGT CCT TAG CC