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. Author manuscript; available in PMC: 2024 Aug 8.
Published in final edited form as: Methods Mol Biol. 2021;2270:437–450. doi: 10.1007/978-1-0716-1237-8_23

Fig. 3.

Fig. 3

Gating strategy for the analysis of IL-35-producing CD138+ B cells. Live cells from LPS-stimulated B220/CD45R cells were stained with an anti-CD138 antibody and sorted by FACS. CD138+ or CD138− cells were then subjected to intracellular cytokine staining analysis. The representative flow cytometry plot shows the percentage of IL-35-expressing CD138+ B cells (p35+ and Ebi3+). Bar chart shows the mean percentage of IL-35 expressing CD138+ or CD138− cells from 10 independent i35-Breg preparations. ****p < 0.0001