|
E. colistrains |
| TOP10 |
F– Φ80lacZΔM15 ΔlacX74 recA1 |
Tiangen |
| BL21(DE3) |
F–ompT hsdSB (rB–mB–) gal dcm (DE3) |
Tiangen |
| DH5α |
F– φ80 lacZΔM15 Δ(lacZYA-argF) |
Tiangen |
| Rosseta(DE3) |
F–ompT hsdSB (rB–mB–) gal dcm (DE3) pRARE
|
Tiangen |
| Plasmids |
| pET-21a |
AmpR, T7 promoter, lac operator |
Merck |
| pET-32a |
AmpR, T7 promoter, lac operator, TrxA tag |
Merck |
| pPmer |
pET-21a derivative containing
natural merR and Pmer divergent
promoter region cloned into BglII and XbaI sites |
(11) |
| pPpbr |
pET-21a derivative containing
a redesigned lead sensory element from Klebsiella pneumoniae CG43 plasmid pLVPK |
this study |
| pPars |
pET-21a derivative
containing
a redesigned arsenic sensory element from Escherichia
coli K12 chromosomal ars operon cloned
into BglII and XbaI sites |
this study |
| pCadR10-DV |
The vioABCE gene
cluster fused downstream of a redesigned cadmium sensory element
from Halomonas caseinilytica JCM 14802 |
(36) |
| pET-HbpAv |
pET-21a derivative containing
the HbpAv coding gene |
this study |
| pET-bFMO |
pET-21a
derivative containing
the bFMO coding gene |
this study |
| pET-cFMO |
pET-21a
derivative containing
the cFMO coding gene |
this study |
| pET-rFPMO |
pET-21a
derivative containing
the rFPMO coding gene |
this study |
| pET-TrxA-bFMO |
pET-32a
derivative containing
the fused TrxA-bFMO coding gene |
this study |
| pET-TrxA-cFMO |
pET-32a derivative containing
the fused TrxA-cFMO coding gene |
this study |
| pHg-bFMO |
pPmer derivative containing
the bFMO coding gene under transcriptional control of Hg(II) sensory
element |
this study |
| pPb-bFMO |
pPb-vioABCE derivative containing
the bFMO coding gene under transcriptional control of Pb(II) sensory
element |
this study |
| pCd-bFMO |
pCadR10-DV derivative containing
the bFMO coding gene under transcriptional control of Cd(II) sensory
element |
this study |
| pHg-TrxA-bFMO |
pPmer derivative containing
the fused TrxA-bFMO coding gene under transcriptional control of Hg(II)
sensory element |
this
study |
| pHg-TrxA-cFMO |
pPmer derivative containing
the fused TrxA-cFMO coding gene under transcriptional control of Hg(II)
sensory element |
This
study |
| pHg-rFPMO |
pPmer derivative containing
the rFPMO coding gene under transcriptional control of Hg(II) sensory
element |
this study |
| pPb-TrxA-bFMO |
pPb-vioABCE derivative containing
the fused TrxA-bFMO coding gene under transcriptional control of Pb(II)
sensory element |
this
study |
| pAs-TrxA-bFMO |
pPars derivative containing
the fused TrxA-bFMO coding gene under transcriptional control of As(III)
sensory element |
this
study |
| pCd-TrxA-bFMO |
pCadR10-DV derivative containing
the fused TrxA-bFMO coding gene under transcriptional control of Cd(II)
sensory element |
this
study |
