Table 2:
Composition of synthetic mixture “spike-in” Standards. Here we show the proportions of which different SARS-CoV-2 lineages were mixed to generate a collection of artificial samples for our benchmark. For example, the sample Mix_01 comprises 25% original Wuhan-Hu-1 A.1 and 75% Alpha B.1.1.7 (
). All samples were sequenced with Ion Torrent and raw data are available under BioProject number PRJNA912560 in the NCBI Sequence Read Archive. Please note that no real wastewater was used to construct these synthetic mixtures because we wanted to reduce any side effects for our gold standard in the context of this study.
| Sample ID | Composition |
|---|---|
| Mix_01 |
|
| Mix_02 |
|
| Mix_03 |
|
| Mix_04 |
|
| Mix_05 |
|
| Mix_06 |
|
| Mix_07 |
|
| Mix_08 |
|
| Mix_09 |
|
| Mix_10 |
|
| Mix_11 |
|
| Mix_12 |
|
| Mix_13 |
|
| Mix_14 |
|
| Mix_15 |
|
| Mix_16 |
|
– Wuhan-Hu-1 A.1; 
– Alpha B.1.1.7; 
– Beta B.1.351; 
– Gamma P.1; 
– Iota B.1.526; 
– Delta B.1.617.2; 
– Delta AY.1; 
– Delta AY.2; 
– Omicron BA.1; 
– Omicron BA.2; 
– Omicron BA.2.5