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. 1992 Mar 15;282(Pt 3):711–714. doi: 10.1042/bj2820711

Occurrence of fatty acid epoxide hydrolases in soybean (Glycine max). Purification and characterization of the soluble form.

E Blée 1, F Schuber 1
PMCID: PMC1130845  PMID: 1554352

Abstract

Epoxide hydrolases catalysing the hydration of cis-9,10-epoxystearate into threo-9,10-dihydroxystearate have been detected in soybean (Glycine max) seedlings. The major activity was found in the cytosol, a minor fraction being strongly associated with microsomes. The soluble enzyme, which was purified to apparent homogeneity by (NH4)2SO4 fractionation, hydrophobic, DEAE- and gel-filtration chromatographies, has a molecular mass of 64 kDa and a pI of 5.4.

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Selected References

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