Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1992 Mar 15;282(Pt 3):711–714. doi: 10.1042/bj2820711

Occurrence of fatty acid epoxide hydrolases in soybean (Glycine max). Purification and characterization of the soluble form.

E Blée 1, F Schuber 1
PMCID: PMC1130845  PMID: 1554352

Abstract

Epoxide hydrolases catalysing the hydration of cis-9,10-epoxystearate into threo-9,10-dihydroxystearate have been detected in soybean (Glycine max) seedlings. The major activity was found in the cytosol, a minor fraction being strongly associated with microsomes. The soluble enzyme, which was purified to apparent homogeneity by (NH4)2SO4 fractionation, hydrophobic, DEAE- and gel-filtration chromatographies, has a molecular mass of 64 kDa and a pI of 5.4.

Full text

PDF
713

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blée E., Schuber F. Efficient epoxidation of unsaturated fatty acids by a hydroperoxide-dependent oxygenase. J Biol Chem. 1990 Aug 5;265(22):12887–12894. [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Croteau R., Kolattukudy P. E. Biosynthesis of hydroxyfatty acid polymers. Enzymatic epoxidation of 18-hydroxyoleic acid to 18-hydroxy-cis-9,10-epoxystearic acid by a particulate preparation from spinach (Spinacia oleracea). Arch Biochem Biophys. 1975 Sep;170(1):61–72. doi: 10.1016/0003-9861(75)90097-1. [DOI] [PubMed] [Google Scholar]
  4. Gill S. S., Hammock B. D. Hydration of cis- and trans-epoxymethyl stearates by the cytosolic epoxide hydrase of mouse liver. Biochem Biophys Res Commun. 1979 Aug 13;89(3):965–971. doi: 10.1016/0006-291x(79)91872-2. [DOI] [PubMed] [Google Scholar]
  5. Guenthner T. M., Oesch F. Identification and characterization of a new epoxide hydrolase from mouse liver microsomes. J Biol Chem. 1983 Dec 25;258(24):15054–15061. [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Lu A. Y., Jerina D. M., Levin W. Liver microsomal epoxide hydrase. J Biol Chem. 1977 Jun 10;252(11):3715–3723. [PubMed] [Google Scholar]
  8. Meijer J., DePierre J. W. Cytosolic epoxide hydrolase. Chem Biol Interact. 1988;64(3):207–249. doi: 10.1016/0009-2797(88)90100-7. [DOI] [PubMed] [Google Scholar]
  9. Morrissey J. H. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem. 1981 Nov 1;117(2):307–310. doi: 10.1016/0003-2697(81)90783-1. [DOI] [PubMed] [Google Scholar]
  10. Orsi B. A., Tipton K. F. Kinetic analysis of progress curves. Methods Enzymol. 1979;63:159–183. doi: 10.1016/0076-6879(79)63010-0. [DOI] [PubMed] [Google Scholar]
  11. Watabe T., Komatsu T., Isobe M., Tsubaki A. Mouse liver microsomal cholesterol epoxide hydrolase: a specific inhibition of its activity by 5,6 alpha-Imino-5 alpha-cholestan-3 alpha-OL. Chem Biol Interact. 1983 Apr-May;44(1-2):143–154. doi: 10.1016/0009-2797(83)90136-9. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES