Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Information of Tetra-Primer ARMS-PCR primers used
| IL-10 SNP | Primer Sequence | Tm (C) AT (C) | Product size (bp) |
|---|---|---|---|
| -1082 G > A | Forward inner primer (A allele): 5¢-AACACTACTAAGGCTTCTTTGGGCAA | 64 62 | A allele: 197 |
| Reverse inner primer (G allele): 5¢-ACTTTCCTCTTACCTATCCCTACTTCACC | 64 | G allele: 288 | |
| Forward outer primer: 5¢-CCAGTTACAGTCTAAACTGGAATGCAG | 64 | 430 (from two | |
| Reverse outer primer: 5¢-CTTGGATTAAATTGGCCTTAGAGTTTCT | 64 | outer primers) |
