Fig 11. Ehvps26 gene knocking down and its effect in trophozoites phagocytosis.

A. Western blot of EhVps26 in control and Ehvps26-knocked down cells (Ehvps26-KD). Control: Trophozoites that were not fed with dsRNA. Bottom: Densitometry of the bands of three independent experiments using α-actin as a loading control. B. Confocal immunofluorescence of EhVps26 in control and Ehvps26-KD using α-EhVps26 antibodies. At right: Immunofluorescence quantification of at least 15 z-stacks of 0.5 μm from independent cells. **P<0.01. C. Rate of phagocytosis of control and Ehvps26-KD cells. The plot is representative of two independent experiments by triplicate. *P<0.05, **P<0.01.