Skip to main content
. Author manuscript; available in PMC: 2024 Aug 8.
Published in final edited form as: Science. 2024 May 17;384(6697):eadj8321. doi: 10.1126/science.adj8321

Fig. 2. BG18 type I lineages and structural analysis.

Fig. 2.

(A) Gene segment assignment and representative HCDR3 sequence of 38 BG18 type I lineages. The D3-41 gene is colored blue and two critical contact residues are colored red. D3-41 in alternate reading frame is colored green. (B)-(E) upper panels show cryo-EM models of (B) BG18_GL0 Fab in complex with N332-GT2 (PDB ID: 6DFH), (C) canonical BG18 type I macaque Fab RM_N332_03 in complex with N332-GT5 (KD, 16 pM), (D) BG18 type I Fab RM_N332_36 with D3-41 in alternate reading frame in complex with N332-GT5 (KD, 38 pM), (E) BG18 type I Fab RM_N332_32 with kappa light chain in complex with N332-GT5 (KD, 81 pM). (B – E) lower panels show magnified view of HCDR3 interacting with gp120 for each structure; gp120 is colored gray and the N332 epitope (N332-GT2 residues Gly324, Asp325, Val326, Arg327, Met328, Ala329, His330, Ile415, Leu416 and Pro417 or the equivalent position in N332-GT5) colored red; HCDR3s are colored blue, orange, purple, green for BG18_GL0, RM_N332_03, RM_N332_36, and RM_N332-32, respectively.