Extended Data Fig. 2 |. The transcriptional induction of lipid biosynthesis following a primary thermogenic response is specific to BAT.

a, Relative gene expression of Ucp1 in iWAT and BAT following transient cold exposure (n = 5 independent animals per condition, except iWAT Day 1 n = 4, iWAT Day 2 n = 2, and iWAT Day 4 n = 4). b, Relative gene expression of Fasn in iWAT and BAT following transient cold exposure (n = 5 independent animals per condition, except iWAT Day 1 n = 4, iWAT Day 2 n = 3, and iWAT Day 4 n = 4). c, Relative gene expression of Fasn in BAT, liver, and muscle from mice in their primary thermogenic response (1cyc) and secondary thermogenic response (2cyc) (n = 5 independent animals per condition). d, Western blot of FASN protein in BAT from cold-naïve mice compared to cold-experienced mice, and relative expression analysis compared to housekeeping gene (Vinculin) (n = 4 independent animals per condition). e, Western blot of UCP1 protein in BAT from cold-naïve mice compared to cold-experienced mice, and relative expression analysis compared to housekeeping gene (Tubulin) (n = 4 independent animals per condition). f, Representative H&E histology images of BAT from cold-naïve mice and cold-experienced mice 4 days after a transient cold exposure (n = 4 independent animals per condition), scale bar = 50μm. Error bars indicate means ± s.e.m. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Exact P values are presented in the source data file for Extended Data Fig. 2.