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Immunofluorescent staining of SV envelope proteins in primary rat neuronal cultures infected with SVN (A and B) or SVNI (C and D) examined 24 h (A and C) or 48 h (B and D) postinfection. Neuronal monolayers were seeded on polylysine-coated glass coverslips and infected at a multiplicity of infection of 1 and then subjected to immunofluorescence analysis as described in Materials and Methods.
