FIGURE 3.

Hypoxia promotes the transcription and the mitochondrial dissociation of HK2. (A, C, D) Immunoblot analyses were performed with the indicated antibodies. (A) PANC‐1 and AsPC‐1 cells were cultured under normoxic or hypoxic condition for the indicated time. (B, C) PANC‐1 and AsPC‐1 cells were cultured under normoxic or hypoxic condition for 24 h in the absence or presence of GN44028 (20 nM). RT‐PCR (B) and immunoblot analyses (C) were performed. N.S., not significant for the indicated comparison; ***p < 0.001. (D) Mitochondrial and cytosolic fractions of PANC‐1 and AsPC‐1 cells cultured under normoxic or hypoxic condition for 24 h were prepared. (E) PANC‐1 and AsPC‐1 cells were stimulated with or without hypoxia for 24 h. Immunofluorescence analyses were performed with an anti‐HK2 antibody. Scale bars, 5 μm.