. 2024 Feb 22;123(14):2085–2096. doi: 10.1016/j.bpj.2024.02.016

Table 1.

Summary of the estimated unligand open probability ( $P_{o \cdot u n l i g a n d e d}$ ) and associated closed-open free energy difference ( ${Δ G}_{u n l i g a n d e d}$ ), DZ-to-pore linkage ( ${Δ Δ G}_{D Z}$ ), and EC₅₀ values for GABA and DZ CRCs for all tested constructs, values are median (25–75% interquartile range) across cells

	$P_{o \cdot u n l i g a n d e d}$	${Δ G}_{u n l i g a n d e d}$ (kcal/mol)	${Δ Δ G}_{D Z}$ (kcal/mol)	GABA EC₅₀ (μM)	DZ EC₅₀ (μM)
α1β2γ2	0^a	N/A	N/A	48 (32–65)	N/A
α_mβ2γ2	0^a	N/A	N/A	410 (220–450)	N/A
α1β_mγ2	0.12 (0.11–0.20)	1.2 (0.9–1.2)	−0.1 (−0.1–(−0.1))	1.2 (1.1–1.3)	0.09 (0.06–0.11)
α_mβ_mγ2	0.07 (0.07–0.12)	1.5 (1.2–1.5)	−0.5 (−0.6–(−0.5))	16 (14–21)	0.34 (0.25–0.42)
α1(L9′T)β2γ2	0.39 (0.33–0.60)	0.3 (−0.3–0.4)	−0.2 (−0.3–(−0.2))	0.24 (0.18–0.37)	0.07 (0.06–0.10)
α_m(L9′T)β2γ2	0.17 (0.14–0.20)	0.9 (0.8–1.1)	−1.1 (−1.3–(−0.9))	0.28 (0.19–0.50)	0.12 (0.07–0.19)
α1(L9′T)β_mγ2	0.68 (0.66–0.70)	−0.4 (−0.5–(−0.4))	−0.3 (−0.4–(−0.2))	2.4 (2.0–2.6)	0.13 (0.07–0.17)
α1(L9′T)β2γ_m	0.16 (0.11–0.29)	1.0 (0.5–1.3)	−0.3 (−0.3–(−0.3))	0.27 (0.25–0.28)	0.07 (0.07–0.09)
α_m(L9′T)β_mγ2	0.64 (0.54–0.66)	−0.3 (−0.4–(−0.1))	−0.9 (−0.9–(−0.6))	1.7 (1.1–2.3)	0.11 (0.08–0.12)
α_m(L9′T)β2γ_m	0.04 (0.04–0.04)	1.9 (1.8–2.0)	−0.7 (−0.9–(−0.6))	0.58 (0.47–0.66)	0.25 (0.13–0.26)
α1(L9′T)β_mγ_m	0.77 (0.72–0.82)	−0.7 (−0.9–(−0.6))	−0.2 (−0.3–(−0.1))	0.62 (0.39–0.92)	0.13 (0.09–0.17)
α_m(L9′T)β_mγ_m	0.51 (0.44–0.56)	0.0 (−0.1–0.1)	−0.4 (−0.4–(−0.3))	1.1 (0.5–2.3)	0.06 (0.02–0.10)

Data for individual cells are plotted in Figs. 6, 7, S7, and S8.

No detectable PTX-sensitive current.