TABLE 1.

Primers used for reverse transcription-quantitative polymerase chain reaction.

Gene	Primer sequence
GAPDH	F: 5′-GCA​AGT​TCA​ACG​GCA​CAG-3′
	R: 5′-GCC​AGT​AGA​CTC​CAC​GAC​AT-3′
IL-1β	F: 5′-CCA​CAG​ACC​TTC​CAG​GAG​AAT​G-3′
	R: 5′-GTG​CAG​TTC​AGT​GAT​CGT​ACA​GG-3′
IL-10	F: 5′- CAT​GCT​GCT​GGG​CCT​GAA-3′
	R: 5′-CGT​CTC​CTT​GAT​CTG​CTT​GAT​G-3′
TNF	F: 5′- GCT​TGT​CCC​TGC​TAC​CCG​C-3′
	R: 5′-GTC​AGG​GGA​TGT​GGC​GTC​T-3′
IL-6	F: 5′- TAG​TCC​TTC​CTA​CCC​CAA​TTT​CC-3′
	R: 5′- TTG​GTC​CTT​AGC​CAC​TCC​TTC-3′
INS	F: 5′- CCT​GTG​GAT​GCG​CTT​CCT​G-3′
	R: 5′-TCC​AGT​TGG​TAG​AGG​GAG​CA-3′
APOE	F: 5′-GCT​CCC​AAG​TCA​CAC​AGG​AA-3′
	R: 5′-TAT​CTG​CTG​GGT​CTG​CTC​CT-3′
APOA1	F: 5′-GGG​AGT​TCT​GGC​AGC​AAG​AT-3′
	R: 5′-TCG​ATC​AGG​GTA​GGG​TGG​TT-3′
LEP	F: 5′- ATG​TGC​TGG​AGA​CCC​CTG​T-3′
	R: 5′- GCA​TTC​AGG​GCT​AAG​GTC​CA-3′
Caspase-1	F: 5′- GGA​AAC​AAA​AGT​CGG​CAG​AG-3′
	R: 5′- ACG​CTG​TAC​CCC​AGA​TTT​TG
NLRP3	F: 5′- ATG​AAC​TCC​TTC​TCC​ACA​AGC​GCC​TTC-3′
	R: 5′-TGG​CTT​GTT​CCT​CAC​TAC​TCT​CAA​ATC-3′
Pro-IL-1β	F:5′- CAC​GAT​GCA​CCT​GTA​CGA​TCA-3′
	R: 5′- GTT​GCT​CCA​TAT​CCT​GTC​CCT-3′
IL-18	F: 5′-ATG GCT GCC ATG TCA GAA GA-3′
	R: 5′- TTG TTA AGC TTA TAA ATC ATG CGG CCT CAG G-3′