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. 2024 Aug 8;14:18426. doi: 10.1038/s41598-024-66600-1

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply, 2024 2024

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Examples of the HiTIPS GUI. (A) Representative screenshot of the HiTIPS GUI for HTI dataset selection and on-demand image loading, with metadata loading and integration in various formats, including CellVoyager and Micro-Manager. (B) Representative screenshot of the image visualization controls in the HiTIPS GUI, including, fluorescence channel toggling and z-projected views for 3D z-stacks, and fluorescence intensity visualization adjustment. (C) Representative screenshot of the overlayed display in the HiTIPS GUI of nuclei masks borders (red) and spot detection in 2 different channels (red and green circles) output from image HiTIPS analysis modules.