Table 1. ICSI/TESE-ICSI outcome using WI rat spermatozoa.
| Sperm source | Sperm cryopreservation |
Sperm suspension | Injected | Survived (%) | Pronuclei-formed (%) |
Transferred | Offspring (%) |
|---|---|---|---|---|---|---|---|
| Epididymal | − | Iso-osmotic | 210 | 172 | 121 | 126 | 18 |
| (81.9 ± 4.5) | (70.3 ± 6.2) a | (14.3 ± 3.4) ab | |||||
| + | Iso-osmotic | 110 | 83 | 65 | 65 | 17 | |
| (75.5 ± 4.2) | (78.3 ± 3.9) a | (26.2 ± 11.3) a | |||||
| Testicular | + | Iso-osmotic | 96 | 80 | 39 | 43 | 2 |
| (83.3 ± 3.9) | (48.8 ± 7.9) b | (4.7 ± 6.0) b | |||||
| + | Hypo-osmotic | 186 | 137 | 79 | 82 | 3 | |
| (73.7 ± 1.8) | (57.6 ± 2.8) b | (3.7 ± 3.5) b | |||||
%: Mean ± SEM. a, b Different superscripts indicate significant differences at P < 0.05.