Table 2.
Outcomes from the PERMANOVA of sample parameters (a) and environmental conditions (b) for the whole-fauna OTU dataset from fixative DNA metabarcoding.
| a | df | SoS | R2 | F | P (> F) | b | df | SoS | R2 | F | P (> F) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Lake | 14 | 47.409 | 0.688 | 44.863 | 0.005 | Area | 1 | 5.037 | 0.073 | 19.510 | 0.034 |
| Day | 2 | 0.250 | 0.004 | 1.654 | 0.066 | Altitude | 1 | 4.721 | 0.069 | 18.285 | 0.041 |
| Reads | 1 | 0.153 | 0.002 | 2.029 | 0.001 | pH | 1 | 6.307 | 0.092 | 24.428 | 0.001 |
| c(DNA) | 1 | 0.155 | 0.002 | 2.058 | 0.023 | NO3-N | 1 | 4.405 | 0.064 | 17.061 | 0.094 |
| c(EtOH) | 1 | 0.110 | 0.002 | 1.456 | 0.199 | Residual | 174 | 45.182 | 0.656 | – | – |
| Residual | 160 | 12.077 | 0.175 | – | – | Total | 179 | 68.881 | 1.000 | – | – |
| Total | 179 | 68.881 | 1.000 | – | – |
df = degrees of freedom, SoS = sum of squares, F = F model ratio, c() = concentration of DNA or ethanol.