Fig. 4.

Ectopic expression of LMO4 enhances transcriptional programs that regulate stemness. a Volcano plot displaying changes in gene expression between pmel-1 Thy1.1⁺ and Lmo4-Thy1.1⁺ CD8⁺ T cells. Gene expression was evaluated by RNA-seq of pmel-1 CD62L^-KLRG1^- T cells collected 5 d after transfer of 10⁵ pmel-1 Thy1.1⁺ and Lmo4-Thy1.1⁺ CD8⁺ T cells into wild-type mice infected with gp100-vv (n = 5 mice/group). b Bubble plot illustrating significantly enriched pathways related to CD8⁺ T cell memory and effector differentiation. c GSEA revealing positive enrichment of genes universally characteristic of stem-like T cells,³³ in pmel-1 Lmo4-Thy1.1⁺ CD8⁺ T cells. d Venn diagram illustrating the overlap between pathways significantly upregulated in Lmo4-Thy1.1⁺ and downregulated in Myb^Δ/Δ pmel-1 CD8⁺ T cells, generated under identical experimental conditions, described in (a). e Bidirectional bar plot displaying the enrichment scores of selected enriched pathways in Lmo4-Thy1.1 and Myb^Δ/Δ pmel-1 CD8⁺ T cells. P-value was calculated with c Kolmogorov–Smirnov test, d Fisher’s Exact Test