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. 2024 Aug 9;9:199. doi: 10.1038/s41392-024-01915-z

Fig. 6.

Fig. 6

STAT3 is essential for LMO4-induced CD8+ T cell stemness and enhanced antitumor responses. a Experimental design to assess the impact of Stat3 versus Thy1.2 (control) deletion on Lmo4-induced stem-like T cell formation. b Flow cytometry histograms showing Thy1.2 (left panel) and STAT3 (right panel) to assess knockout efficiency compared to controls in pmel-1 CD8+ T cells. c, d Flow cytometry analysis (c) and percentages (d) of splenic pmel-1 CD8+ T cells 5 d after transfer of either 105 pmel-1 Ly5.1+ Thy1.1+Thy1.2KO, Thy1.1+Stat3KO, Lmo4-Thy1.1+Thy1.2KO or Lmo4-Thy1.1+Stat3KO CD8+ T cells into wild-type mice infected with gp100-vv (five mice per group). e, f Flow cytometry analysis (e) and percentages (f) of CD62L+CD44+ splenic pmel-1 T cells 30 d after transfer as in (c, d). g, h Tumor size (g) and survival curve (h) of B16KVP tumor-bearing wild-type mice after transfer of either 3.5 × 105 pmel-1 Thy1.1+Thy1.2KO, Thy1.1+Stat3KO, Lmo4-Thy1.1+Thy1.2KO or Lmo4-Thy1.1+Stat3KO CD8+ T cells into wild-type mice treated with gp100-vv and IL-2. *P < 0.05, **P < 0.01 (d, f, unpaired two-tailed Student’s t-test, g, Wilcoxon rank-sum test; h, log-rank (Mantel–Cox) test). (a) was created with BioRender.com