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Standard curves for QF-PCR assays for VZV genes encoding ORFs 29 and 62 and gB. Shown are the Ct values at which a given input of a VZV DNA-containing plasmid was detected by PCR. Plasmid concentrations tested ranged from 100 to 106 copies per reaction mixture. The Spearman rank coefficient of correlation (r) for lines fitting each standard curve are given.
