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. 2024 Aug 9;4(8):1991–2007. doi: 10.1158/2767-9764.CRC-24-0128

Figure 6.

Figure 6

p53R172H and p53R245W interact with Nr5a2 to alter transcription. A, Western blot analyses of p53R172H and p53R245W protein levels in Trp53-null breast tumor cells infected with lentiviral vectors expressing p53R172H or p53R245W, compared with empty vector control. B, qRT-PCR analysis of p53R172H downstream target genes Argef18, Fbxo2, Lime1, Msi1, Nrtn with Nr5a2 motifs after lentivirus infection of p53R172H (n = 6, P value <0.002, 0.01, 0.002, 0.002, 0.03, respectively, Mann–Whitney U test). C, qRT-PCR analysis of p53R245W downstream target genes Add2, Ankrd2, Myh4, and Prss35 with Nr5a2 motifs after lentivirus infection of p53R245W (n = 6, P value < 0.002, 0.4, 0.04, 0.6, 0.2, respectively, Mann–Whitney U test). C, control. D, qRT-PCR analysis of p53R245W downstream target genes Add2, Ankrd2, and Myh4 after siRNA-mediated knockdown of Nr5a2 in MaPR245W/− cell line, RM0014. C, control. E, Co-immunoprecipitation experiments of MaPR172H/− cell line YZ10 and MaPR245W/− cell line JM1564 using Nr5a2 pull-down and probing for mutant p53.