Table 1.

The efficacy of the thieno-pyrimidin-4-yl-hydrazylidene (TPH) derivatives, TPH104c and TPH104m, in the TNBC cell line, BT-20, MDA-MB-231, and MDA-MB-468, and the normal human mammary epithelial cell lines HMEC, MCF-10A and MCF-12A.

Compounds	IC50 ± SD (μM)
	TNBC			Normal
	BT-20	MDA-MB-231	MDA-MB-468	HMEC	MCF-10A	MCF-12A
TPH104c	0.22 ± 0.06	0.48 ± 0.16	0.45 ± 0.17	6.74 ± 0.97	5.84 ± 1.81	6.04 ± 1.56
TPH104m	0.18 ± 0.03	0.47 ± 0.15	0.27 ± 0.14	5.67 ± 0.23	5.24 ± 2.47	6.13 ± 1.30

Cell survival assay was performed using the MTT assay. IC₅₀ values represent the concentration required to inhibit cell proliferation by 50%. These values are presented as the average ± SD of three separate experiments conducted in triplicate. The efficacy of TPH104c and TPH104m was determined in TNBC cell lines: BT-20, MDA-MB-231, MDA-MB-468, and normal mammary epithelial cell lines: primary HMEC, MCF-10A, and MCF-12A.