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NMOSD patient-derived IgGs bound to both human AQP4 M23 and rat AQP4 M23 but did not bind to human AQP4 M1. (a–c) Binding of NMO1-IgG (a), NMO2-IgG (b), and NMO3-IgG (c) to rat M23 (blue), human M23 (red), and human M1 (green) expressed in CHO-K1 cells evaluated by enzyme-linked immunosorbent assay (ELISA). Values are mean ± SEM of four independent experiments.
