Figure 7.

Immuno-blot analysis of the proteins involved in wheat treated with PDS solution under salt stress. Proteins extracted from wheat root and leaf were separated on 10% SDS-polyacrylamide gel by electrophoresis and transferred onto membranes. The membranes were cross-reacted with anti-ascorbate peroxidase (A) and H⁺-ATPase (B) antibodies. A staining pattern with Coomassie brilliant blue was used as a loading control (Figure S2). The integrated densities of the bands were calculated using ImageJ software. The data are presented as mean ± SD from three independent biological replicates (Figures S3 and S4). Student’s t-test was used to compare values between two groups. Asterisks indicate significant changes (*, p ≤ 0.05; **, p ≤ 0.01).