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. 2024 Jul 25;25(15):8121. doi: 10.3390/ijms25158121

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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COL6A3 expression in EOC cell lines and derived exosomes. (A,B) COL6A3 was expressed in ES2 derivatives, SKOV3/COL6A3, and MSC-OCSPC-derived-exosomes and lysates, while there was no expression in SKOV3- and ES2/shRNA-derived exosomes and cell lysates. (C) The CD9 and CD63 representative exosome markers were seen in ES2-derivative-, SKOV3/COL6A3-, and MSC-OCSPCs-derived exosomes, but CD9 and CD63 were not detected in those cell lysates. (D) Immunostaining of COL6A3 was positive in ovarian serous carcinoma stromal cells, which surrounded cancer cells with negative staining.