Table 1. Requirements for Resolving and Quantifying hTTR Tetramers Containing mTTR and wt-TTR Subunits.
| mutant | average monomer mass (Da) | average tetramer mass (Da) | Δmass of tetramer from wt-TTR (Da) | Δmass of monomer from wt-TTR (Da) | Δm/z shift for each hTTR state (16+ CS) | resolving power needed to separate hTTR tetramers |
|---|---|---|---|---|---|---|
| WT | 13906 | 55622 | NA | NA | NA | NA |
| L55P | 13889 | 55558 | –64.20 | –16.05 | –1.00 | 3462 |
| V30M | 13938 | 55750 | 128.24 | 32.06 | 2.00 | 1739 |
| F87A | 13829 | 55318 | –304.40 | –76.10 | –4.76 | 727 |
| T119M | 13936 | 55742 | 120.32 | 30.08 | 1.88 | 1853 |