Figure 2.

Validation of primer sets using SARS-CoV-2 synthetic RNA. Real-time RT-LAMP assays were performed on viral genomic RNA (1 × 10⁴ to 1 × 10¹ genome copies µL⁻¹) to construct curves for the orf1ab and N15 regions to tabulate PCR amplification efficiency. Time to positivity (Tp) for each primer set is indicated. Error bars show the standard deviation.