Table 1.
Sensitivity performance expressed as percentage of positive samples detected over 40 cycles at four concentrations between nCoV_IP4-14095 and 2019-nCoV_N2 primer–probe sets.
| Standard Curve Points | ||||||
|---|---|---|---|---|---|---|
| RT Methodology | Gene | 104 | 103 | 102 | 101 | NTC |
| 2-step (6-mers primers) | orf1ab | 10/10 (100%) | 8/8 (100%) | 7/9 (77.78%) | 7/10 (70%) | 0/10 (0%) |
| 3′ N | 10/10 (100%) | 4/10 (44.5%) | 6/10 (60%) | 0/10 (0%) | 0/10 (0%) | |
| 1-step (specific primers) | orf1ab | 9/9 (100%) | 11/16 (68.7%) | 6/9 (66.6%) | 4/10 (40%) | 1/10 (10%) |
| 3′ N | 9/10 (90%) | 8/9 (88.9%) | 8/10 (80%) | 6/10 (60%) | 0/10 (0%) | |