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. 2024 Jul 31;16(15):2725. doi: 10.3390/cancers16152725

Figure 1.

Figure 1

Forward genetic screen using piggyBac transposon identifies Arid1a as a tumor suppressor gene. (a) Schematic of PBonc design, random genomic integration of transposable elements from Rosa26 locus via Cre-lox mediated recombination (PBIRL/PBIRR = piggyBac Inverted Repeat sequences left/right, SA/SD = Splice Acceptor/Donor, PBase = piggyBac transposase, IRES = Internal Ribosomal Entry Sites, Luc = Luciferase). (b) Histology of tamoxifen-induced skeletal tumors harvested from piggyBac inserted cohort shows similar appearance with osteosarcoma (osteoid matrix = bright pink, scale bar 100 and 10 μm). (c) Kaplan–Meier survival analysis of mice with (red) piggyBac mutagenesis showed a 5-month survival disadvantage compared to the ones without (blue); median overall survival was 10.0 months and 15.0 months, respectively (p-value < 0.0001).