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. 2024 Jul 24;16(15):2636. doi: 10.3390/cancers16152636

Figure 2.

Figure 2

GH elevates ABC efflux pump levels in melanoma tumor-derived exosomes and corresponding recipient cells. (A) Protein levels of ABCC1, ABCC2, ABCB1, and ABCG2 in exosomes isolated from human melanoma cells, Malme-3M, SK-Mel-28, and SK-MEL30, 96-h post treatment with 50 ng/mL GH independently or in combination with EC50 dosage of doxorubicin, 500 nM pegvisomant. (B–E) Blots were quantified using ImageJ (version 1.8.0_345) and protein levels were normalized using β-actin as a control and presented as relative protein expression. Blots from the three independent experiments are presented as the mean ± SD and p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***). (F) Protein levels of ABCC1, ACCB1, and ABCG2 in human melanoma cells: Malme-3M, SK-Mel-28, and SK-MEL30, treated with 20 μg/mL exosomes from abovementioned melanoma cells. (G–I) Blots were quantified using ImageJ and expressions were normalized against β-actin and presented as relative protein expression. Blots from the three independent experiments are presented as the mean ± SD and p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***).