Figure 3.

Blocking autocrine/paracrine GH action in melanoma suppresses ABC efflux pumps in tumor-derived exosomes. (A) Protein levels of ABCC1 and ABCB1 in exosomes isolated from human melanoma cells, Malme-3M, SK-Mel-28, and SK-MEL30, 96-h post treatment with 500 nM pegvisomant and an EC50 dosage of doxorubicin independently or in combination. (B,C) Blots were quantified using ImageJ (version 1.8.0_345) and expressions were normalized against β-actin and presented as relative protein expression. Blots from the two independent experiments are presented as the mean ± SD and p < 0.05 (*).