Table 2.

Tests used to determine miRNA expression.

Reference	miRNA Expression Method	Commercial Kits Used
[32]	LNA qRT-PCR	Aurum Total RNA mini kit (BioRad, Hercules, CA, USA) miRCury LNA Universal RT microRNA PCR Kit (Qiagen, Hilden, Germany) miRCury LNA miRNA PCR assays (Qiagen, Hilden, Germany)
[33]	SYBR Green qRT-PCR	PAXgene Tissue miRNA Kit (Qiagen, Hilden, Germany) miScript Reverse Transcription Kit (Qiagen, Hilden, Germany) SYBR® Green PCR Kit (Qiagen, Hilden, Germany)
[34]	Taqman probe qRT-PCR	TaqMan Advanced miRNA Assays (Applied Biosystems, Waltham, MA, USA) TaqMan Advanced miRNA cDNA Synthesis Kit (Applied Biosystems, Waltham, MA, USA) CFX96 Touch Real-Time PCR Detection System (BioRad, Hercules, CA, USA)
[35]	LNA-ISH	miRCury LNA miRNA Detection FFPE (Exiqon, Vedbaek, Denmark) microRNA ISH Optimization Kit2 (Exiqon, Vedbaek, Denmark)

Abbreviations: qRT-PCR—quantitative real-time reverse transcription polymerase chain reaction; ISH—in situ hybridization; LNA—locked nuclei acid.