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Synaptic vesicles and calcium channel levels of young and ageing EDL muscles. (A), Western blot analysis of different key presynaptic molecules related to synaptic vesicles. Note that no change was found in any protein analysed. (B), Western blot analysis of the P/Q-type VGCC, showing a significant decrease in the protein levels between young and ageing muscles. Data are represented as means ± SD. Statistical significance was determined using the Mann–Whitney test (* p value < 0.05).
