Figure 2.
Expression and concentration of GFAP, Tubulin-IIIβ, and GFAP/Tubulin-IIIβ ratio in the brain in the 1 M study. Subfigures (A–C) represent gene expression calculated using the 2−ΔΔCT method and normalized to GAPDH. Subfigures (D–F) represent protein concentration. The data are represented as mean + SD; p-value < 0.05 is considered significant (*). The sample sizes are indicated below each bar graph. Abbreviations: PPA (propionic acid), GFAP (glial fibrillary acidic protein), SD (standard deviation), 1 M (1 month), 5 M (5 months).