Table 1.
Content of polyphenolic compounds in the M. chamomilla extract.
| Substance | Retention Time, min | Content in the Extract | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Gch [7] | Gch-Arg | Gch-Phe | Gch-β-Ala | Gch-Gly | Gch-Val | Gch-Lys | |||
| UPLC-MS/MS, µg/g of dry extract | |||||||||
| Neochlorogenic acid | 2.72 | 444.94 ± 20.16 | 329.21 ± 4.47 | 322.26 ± 8.51 | 373.81 ± 7.33 | 385.18 ± 13.11 | 382.70 ± 27.35 | 359.65 ± 12.79 | |
| Luteolin | 7.15 | 310.93 ± 22.73 | 270.78 ± 15.81 | 320.87 ± 9.72 | 283.67 ± 8.20 | 309.48 ± 12.86 | 320.93 ± 27.22 | 301.31 ± 6.67 | |
| Isoquercitrin | 5.49 | 921.16 ± 85.20 | 774.55 ± 41.11 | 850.92 ± 85.24 | 872.54 ± 34.08 | 880.89 ± 79.41 | 894.15 ± 43.80 | 838.97 ± 25.75 | |
| Cryptochlorogenic acid | 3.86 | 80.74 ± 13.48 | 62.26 ± 9.76 | 70.97 ± 10.16 | 58.20 ± 5.03 | 62.47 ± 5.47 | 75.10 ± 20.95 | 55.98 ± 12.71 | |
| Luteolin-4-O-glucoside | 6.03 | 45.11 ± 3.67 | 37.98 ± 1.88 | 49.59 ± 4.86 | 44.06 ± 3.86 | 41.68 ± 3.82 | 49.25 ± 6.75 | 43.68 ± 6.82 | |
| Chlorogenic acid | 3.98 | 11,742.31 ± 376.34 | 8984.21 ± 397.30 | 9532.56 ± 179.37 | 10,341.94 ± 211.50 | 10,567.30 ± 220.17 | 10,014.55 ± 167.55 | 9342.78 ± 355.66 | |
| Quercetin | 7.17 | 172.15 ± 12.01 | 138.19 ± 1.54 | 162.89 ± 8.50 | 149.96 ± 6.55 | 156.29 ± 6.19 | 149.27 ± 17.77 | 141.29 ± 6.20 | |
| Isorhamnetin-3-O-rutinoside | 5.86 | 15.40 ± 1.60 | 12.03 ± 1.51 | 11.58 ± 1.28 | 12.78 ± 1.05 | 15.18 ± 1.55 | 14.68 ± 0.98 | 12.94 ± 1.50 | |
| Isorhamnetin-3-glucoside | 6.12 | 410.75 ± 52.07 | 355.44 ± 24.71 | 402.53 ± 13.93 | 385.13 ± 32.20 | 448.75 ± 38.82 | 429.60 ± 16.85 | 406.41 ± 15.58 | |
| Luteolin-3,7-diglucoside | 5.02 | 20.72 ± 1.88 | 15.60 ± 0.98 | 24.16 ± 0.70 | 19.03 ± 4.42 | 21.86 ± 1.36 | 20.04 ± 2.27 | 22.53 ± 6.19 | |
| Vanillic acid | 4.22 | 86.58 ± 5.54 | 64.91 ± 5.23 | 69.80 ± 7.89 | 90.63 ± 2.98 | 88.66 ± 3.46 | 79.04 ± 3.04 | 85.28 ± 12.40 | |
| Caffeic acid | 4.39 | 43.04 ± 3.22 | 40.58 ± 3.01 | 45.80 ± 6.49 | 47.91 ± 7.11 | 37.44 ± 4.50 | 36.52 ± 6.60 | 45.18 ± 4.35 | |
| 3,4-Dihydroxyphenylacetic acid | 2.34 | 184.05 ± 13.38 | 149.40 ± 12.77 | 145.05 ± 5.55 | 180.78 ± 11.94 | 154.06 ± 3.07 | 170.92 ± 4.40 | 151.33 ± 8.57 | |
| Isorhamnetin | 7.95 | 125.32 ± 12.71 | 92.98 ± 5.25 | 116.50 ± 7.41 | 106.09 ± 5.58 | 101.81 ± 1.42 | 112.95 ± 3.76 | 102.56 ± 5.78 | |
| Apigenin | 7.70 | 578.65 ± 63.91 | 462.21 ± 23.88 | 537.43 ± 47.49 | 506.71 ± 11.63 | 502.22 ± 3.14 | 549.22 ± 26.09 | 493.34 ± 29.54 | |
| Kaempherol-3-O-glucoside | 5.94 | 50.76 ± 2.10 | 40.41 ± 3.67 | 49.94 ± 3.18 | 50.78 ± 5.32 | 55.22 ± 3.52 | 58.84 ± 2.27 | 48.15 ± 2.58 | |
| Rutin | 5.39 | 126.49 ± 5.73 | 104.66 ± 3.94 | 102.88 ± 7.79 | 113.02 ± 14.86 | 109.13 ± 10.54 | 107.17 ± 10.24 | 99.26 ± 7.71 | |
| Hyperoside | 5.44 | 366.82 ± 21.21 | 308.22 ± 21.78 | 315.79 ± 16.66 | 357.39 ± 12.20 | 387.16 ± 16.43 | 345.88 ± 21.19 | 347.21 ± 19.20 | |
| Luteolin-7-O-glucoside | 5.56 | 1061.82 ± 83.68 | 874.36 ± 62.18 | 975.10 ± 57.44 | 1031.03 ± 73.93 | 1094.50 ± 72.63 | 1021.56 ± 59.44 | 1070.74 ± 37.66 | |
| 4.5-Dicaffeoylquinic acid | 5.70 | 4912.17 ± 416.85 | 3844.32 ± 149.55 | 4375.54 ± 208.35 | 4320.08 ± 175.34 | 4671.16 ± 242.58 | 4336.20 ± 352.82 | 4227.72 ± 365.27 | |
| 3.5-Dicaffeoylquinic acid | 6.07 | 2512.69 ± 213.23 | 1966.47 ± 76.50 | 2238.19 ± 106.57 | 2209.83 ± 89.69 | 2389.41 ± 124.08 | 2218.07 ± 180.47 | 2162.58 ± 186.85 | |
| 3.4-Dicaffeoylquinic acid | 5.88 | 5152.17 ± 437.22 | 4032.15 ± 156.86 | 4589.32 ± 218.53 | 4531.15 ± 183.91 | 4899.39 ± 254.43 | 4548.06 ± 370.05 | 4434.28 ± 383.12 | |
| Spectrophotometry, % | |||||||||
| Total polyphenolic compounds | 9.70 ± 0.52 | 7.87 ± 0.41 | 8.50 ± 0.34 | 9.40 ± 0.62 | 7.43 ± 0.59 | 8.29 ± 0.53 | 7.49 ± 0.09 | ||
| Hydrocinnamic acids | 3.47 ± 0.15 | 2.75 ± 0.29 | 3.28 ± 0.33 | 3.20 ± 0.14 | 2.64 ± 0.21 | 2.94 ± 0.31 | 2.50 ± 0.32 | ||
| Flavonoids | 9.92 ± 0.32 | 7.16 ± 0.38 | 8.50 ± 0.37 | 7.95 ± 0.09 | 7.55 ± 0.32 | 7.71 ± 0.77 | 7.26 ± 0.57 | ||
Notes: Gch—the dry M. chamomilla extract, obtained with 70% aqueous ethanol solution, and its amino acid preparations with arginine (Gch-Arg), phenylalanine (Gch-Phe), β-alanine (Gch-β-Ala), glycine (Gch-Gly), valine (Gch-Val), and lysine (Gch-Lys).