Skip to main content
. 2024 Jul 24;13(15):1242. doi: 10.3390/cells13151242

Figure 3.

Figure 3

Functionality of the TISU 5′ UTR in methyl-1 pseudoU modified mRNA. (A,B) Luciferase activity in HEK-293 cells (A) and in human blood PBMCs (B) 24 h after transfection of methyl-1 PseudoU modified mRNAs containing no 5′UTR, a Kozak 5′UTR, or a TISU 5′ UTR and coding for either gaussia luciferase (white bars) or firefly luciferase (grey bars). (C) Fluorescence over 48 h in HEK-293 cells after transfection of methyl-1 PseudoU mRNAs containing no 5′UTR, a Kozak 5′UTR, or a TISU 5′ UTR and coding for ZsGreen. (A–C) Experiments were performed in triplicate and data are presented as the mean ± SD. One-way ANOVA with Tukey’s multiple comparison test was used to determine the significance between the different groups. ns = not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001.