Skip to main content
. 2024 Jul 24;13(15):1242. doi: 10.3390/cells13151242

Figure 4.

Figure 4

Comparison of minRNA and optimized mRNAs in translating protein. (A) Schematic representation of the optimized (“opt”) and minimized (“min”) mRNAs. Depicted are the 5′ and 3′ UTRs of opt mRNA and the coding sequence (CDS) of both. minRNA lacks the conventional 5′ and 3′ UTRs and contains the 4-nucleotide-long TISU Translation Initiator of Short 5′ UTR (TISU) element. (B,C) Luciferase activity 24 h after transfection of min and opt mRNAs coding for (B) gaussia luciferase or (C) firefly luciferase in HEK-293 cells (left panels) and PBMCs (right panels). The mRNAs were unmodified or had full replacement of U residues by modified uracils (PseudoU “ψ”, methyl-1 Pseudo U “m1ψ”, or methoxy U “moU”). (D) Fluorescence in HEK-293 cells over 48 h after transfection of unmodified or methyl-1 pseudoU modified mRNAs coding for ZsGreen. (B,D) Experiments were performed in triplicate and data are presented as the mean ± SD. (B,C) Two-way or (D) one-way ANOVA with Tukey’s multiple comparison test was used to determine the significance between the different groups. ns = not significant, * p < 0.05, *** p < 0.001.