Table 1.
Selected toxins for cell-free expression. The table shows toxins selected to explore the potential of cell-free protein production in venom biodiscovery. Given are the toxin name, ID and/or sources, producing organism and its size. Full data of each toxin, including its sequence, are given in Supplementary Table S1.
| Toxin Group | Toxin Name | UniProt ID or Source | Source Organism | Lineage | Size [kDa] |
|---|---|---|---|---|---|
| Cysteine-rich neurotoxins |
U-Asilidin(1)-Mar1a | P0DQI8 | Machimus arthriticus (Zeller, 1840) | Robber fly | 3.1 |
| U-Asilidin(1)-Eru1a | P0DQJ1 | Eutolmus rufibarbis (Meigen, 1820) | Robber fly | 3.1 | |
| Delta-miturgitoxin-Cp1a | C0HKG7 | Cheiracanthium punctorium (Villers, 1789) | Spider | 15.1 | |
| Omega Hexatoxin Hi1a | P0C2L5 | Hadronyche infensa (Hickman, 1964) | Spider | 3.9 | |
| Omega-theraphotoxin-Cc1a | D5J6X1 | Pelinobius muticus (Karsch, 1885) | Spider | 4.3 | |
| U11-pisautoxin-Dm1a | S5MK94 | Dolomedes mizhoanus (Kishida, 1936) | Spider | 6.8 | |
| U18-barytoxin-Tl1a | W4VRU3 | Trittame loki (Raven, 1990) | Spider | 7.5 | |
| U1-barytoxin-Tl1a | W4VRV2 | Trittame loki (Raven, 1990) | Spider | 11.1 | |
| U1-oxotoxin-Ot1a | W0LQ84 | Oxyopes takobius (Andreeva & Tyschchenko, 1969) | Spider | 13.7 | |
| U1-pisautoxin-Dm1a | S5N3Q8 | Dolomedes mizhoanus (Kishida, 1936) | Spider | 11.4 | |
| U1-TRTX-Lp1b | P61506 | Lasiodora parahybana (Mello-Leitao, 1917) | Spider | 5.7 | |
| Protease inhibitor | Hirudin | P28509 | Hirudo medicinalis (Linnaeus, 1758) | Leech | 7.0 |
| Antimicrobial peptide | Lycotoxin | B6DD06 | Lycosa singoriensis (Laxmann, 1770) | Spider | 4.8 |
| (Putative) Enzyme | Phospholipase D | A0A1L4BJ98 | Hemiscorpius lepturus (Peters, 1861) | Scorpion | 33.0 |
| Phospholipase D | Q8I914 | Loxosceles laeta (Nicolet, 1849) | Spider | 32.1 | |
| Phospholipase D | Q1KY80 | Loxosceles laeta (Nicolet, 1849) | Spider | 32.0 | |
| Phospholipase D | Q1KY79 | Loxosceles laeta (Nicolet, 1849) | Spider | 32.6 | |
| Phospholipase D | C0JB23 | Loxosceles laeta (Nicolet, 1849) | Spider | 31.5 | |
| Phospholipase D | Q8I912 | Loxosceles laeta (Nicolet, 1849) | Spider | 31.7 | |
| CAP | [25] | Argiope bruennichi (Scopoli, 1772) | Spider | 45.3 | |
| Three-finger toxin (3Ftx) | Long neurotoxin 1 | P34074 | Naja annulata (Peters, 1876) | Snake | 7.8 |
| Delta-elapitoxin-Cb1a | P0DL82 | Calliophis bivirgatus (Boie, 1827) | Snake | 6.7 | |
| Scutelatoxin | Q4VRI0 | Oxyuranus s. scutellatus (Peters, 1876) | Snake | 6.6 | |
| Cytotoxin 1 | P01455 | Naja annulifera (Peters, 1854) | Snake | 6.7 | |
| Cytotoxin sagitoxin | P83345 | Naja sagittifera (Wall, 1913) | Snake | 6.8 | |
| Others | Pimplin | Q8WPC8 | Pimpla hypochondriaca (Retzius, 1783) | Wasp | 13.1 |
| Ryncolin-1 | D8VNS7 | Cerberus rynchops (Schneider, 1799) | Snake | 36.4 | |
| Ryncolin-2 | D8VNS8 | Cerberus rynchops (Schneider, 1799) | Snake | 36.6 | |
| Ryncolin-3 | D8VNS9 | Cerberus rynchops (Schneider, 1799) | Snake | 36.4 | |
| Ryncolin-4 | D8VNT0 | Cerberus rynchops (Schneider, 1799) | Snake | 36.2 |