Fig. 8.

The P38-MAPK/NF-κB signaling pathway was involved in LOX-1/ox-LDL -induced cartilage endplate cells senescence. A, B Western blotting and quantitative analysis were performed to assess the expression levels of pathway-related proteins following ox-LDL treatment. C, D Western blotting and quantitative analysis were conducted to evaluate the expression levels of pathway-related proteins after treatment with ox-LDL and siLOX-1/siNC. E The results of immunofluorescence experiment also indicated that ox-LDL treatment could promote the nuclear translocation of NF-κB. F siLOX-1 effectively inhibited the promoting effect of ox-LDL on nuclear translocation of NF-κB. Compared to siNC/ control group, **p < 0.01, ***p < 0.001, ns indicated non-significance difference; Compared to siNC + ox-LDL group, *p < 0.05, **p < 0.01, ns indicated non-significance difference