Figure 4.

Effects of EGb 761 on CCL2 expression (A) and synthesis (B) in LPS-stimulated BV2 cells. Cells were stimulated as described in Materials and Methods. (A) After 4 h of stimulation, RNA was isolated and expression of CCL2 was determined using qPCR. (B) After 24 h of stimulation, supernatants were collected and the release of CCL2 was measured by ELISA. Values are presented as the mean ± SD of six independent experiments. Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc tests with * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 compared to LPS.