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. 2024 Jul 25;13(15):1253. doi: 10.3390/cells13151253

Figure 5.

Figure 5

Defective mitochondrial dynamics in nephrocytes impaired endocytic membrane trafficking: (A–F) Endocytic membrane trafficking in Drosophila nephrocytes (female, 4-day-old) overexpressing (OE) or inhibiting (RNAi; IR) Pink1, park or Marf. Control, (A) Dot > Rab5-YFP, (B) Dot > Rab7-YFP (C) Dot > Rab11-YFP. (A) Early endosome visualized by Rab5-YFP (green) in nephrocytes. Scale bar = 5 µm. Boxed areas are shown magnified to the right (scale bar: 1 μm). (B) Quantitation of Rab5-YFP fluorescence in Pink1/park/Marf-OE/IR nephrocytes. (C) Late endosomes visualized by Rab7-YFP (green) in nephrocytes. Scale bar = 5 µm. Boxed areas are shown magnified to the right (scale bar: 1 μm). (D) Quantitation of Rab7-YFP fluorescence in Pink1/park/Marf-OE/IR nephrocytes. (E) Recycling endosomes visualized by Rab11-YFP (green) in nephrocytes. Scale bar = 5 µm. Boxed areas are shown magnified to the right (scale bar: 1 μm). (F) Quantitation of Rab11-YFP fluorescence in Pink1/park/Marf-OE/IR nephrocytes, relative to control. N = 30 nephrocytes from 6 flies, per group per assay. Results presented as mean ± SD; statistical significance: *, p < 0.05.