Skip to main content
. 2000 Nov 1;28(21):4306–4316. doi: 10.1093/nar/28.21.4306

Figure 1.

Figure 1

Figure 1

αCP2 binds to the collagen α1(I) 3′-UTR. (A) In vitro transcribed RNA labeled by [α-32P]UTP incorporation was incubated with either no extract (lane 1), 40 ng recombinant GST–αCP2 (lane 2), 20 ng recombinant Factor Xa-cleaved GST–αCP2 (lane 3), 10 µg NIH 3T3 cytoplasmic lysate (lane 4) or 10 µg cytoplasmic lysate from activated human hscs (lane 5). The extracts were incubated on ice for 30 min with 11 000 c.p.m. (∼10 pmol) of probe in a total volume of 25 µl and electrophoresed on a 6% native acrylamide gel. The gel was dried and exposed to film. (B) Specific antibodies were used to supershift αCP1 (lane 4) or αCP2 (lane 5), while an antibody to smooth muscle actin did not cause a shift (lane 3). Binding reactions were done as in (A). Antibodies were generous gifts from R. Andino and M. Czyzyk-Krzeska.