Table 1. Oligonucleotide sequences of single-stranded probes.
Probe | Sequence |
---|---|
Wild-type 3′-UTR RNA | 5′-UCC CUC CCA CCC AGC CCA CUU UUC CCC AAC CCU-3′ |
CONS DNA | 5′-TCC CTC CCA CCC AGC CCA CTT TTC CCC AAC CCT-3′ |
CONS+SL DNA | 5′-GCGTGT TCC CTC CCA CCC AGC CCA CTT TTC CCC AAC CAC ACG C-3′ |
CONS DNA D1 | 5′-TCA CTC CCA CCC AGC CCA CTT TTC ACC AAC ACT-3′ |
CONS DNA D3 | 5′-TCC CTC CCA CAC AGC CCA CTT TTC ACC AAC ACT-3′ |
CONS DNA D5 | 5′-TCC CTC CCA CCC AGC CCA CTT TTC ACC AAC CCT-3′ |
CONS DNA D6 | 5′-TCC CTC CCA CCC AGC CCA CTT TTC CCC AAC ACT-3′ |
CONS DNA D356 | 5′-TCC CTC CCA CAC AGC CCA CTT TTC ACC AAC ACT-3′ |
S40 (truncated recognition sequence and stem) | 5′-GCG TGT TCC CTC CCA AGC CCA CTT TTC CCC AAC CAC ACG C-3′ |
S28 (truncated recognition sequence) | 5′-TCC CTC CCA (CCC) AGC CCA CTT TTC CCC AAC C-3′ |
Poly(C) | 5′-CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC-3′ |
Poly(G) | 5′-GGG GGG GGG GGG GGG GGG GGG GGG GGG GGG GGG-3′ |
Mutant 2 | 5′-TCC CTC CCA CCC AGC CCA GAA AAG ATC TGG CCT-3′ |
Sequences in bold are stem–loop sequences. Underlined nucleotides indicate the C→A mutations used in EMSAs.