Figure 8.

Isolation of TS RNP complexes in human colon cancer cells transfected with U6 heterologous plasmids. Human colon cancer H630 cells were transiently transfected in the absence (lane 1) or presence of pGEMmU6 (lane 2) or pGEMmU6/TS:N25 (lane 3). Whole cell extracts were immunoprecipitated with an anti-TS monoclonal antibody and TS RNP complexes were isolated as described in Materials and Methods. PCR products were resolved by electrophoresis on a 1.0% agarose gel stained with ethidium bromide.