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. 2000 Nov 1;28(21):4266–4274. doi: 10.1093/nar/28.21.4266

Figure 9.

Figure 9

Effect of TS:N25 RNA on luciferase expression in HCT-C18 and HCT-C:His-TS+ cells. Human colon cancer HCT-C18 (open square) and HCT-C:His-TS+ (hatched square) cells were transiently transfected with p644, p644/TS:N25 or p644/TS:N25(AS). Luciferase activity was measured 48 h post-transfection using the dual luciferase assay system as described in Materials and Methods. The activity in cells transfected with the p644 plasmid was taken as 100%. Transfection with p644/TS:N25 resulted in a significant increase in luciferase activity (P < 0.01), as indicated by the asterisk, when compared to transfection with p644. Statistical analysis was performed using the paired Student’s t-test. Each value represents the mean ± SE of between three and five experiments.